Application | Comment | Organism |
---|---|---|
food industry | the application of the novel ochratoxin A hydrolytic enzyme to reduce the ochratoxin A contents on some food or feed products is under evaluation | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
ochratoxin A | - |
Aspergillus niger | |
1.1 | - |
ochratoxin A | - |
Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the enzyme is purified from the culture medium by acetone precipitation and Macro-Prep High Q anion exchange chromatography | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
carboxypeptidase A, pH 5.6 | Aspergillus niger |
2.8 | - |
Aspergillus niger extract, pH 5.6 | Aspergillus niger |
2.8 | - |
carboxypeptidase A, pH 7.5 | Aspergillus niger |
9.2 | - |
Aspergillus niger extract, pH 8.5 | Aspergillus niger |
10.8 | - |
carboxypeptidase A, pH 8.5 | Aspergillus niger |
14.4 | - |
culture filtrate | Aspergillus niger |
33.5 | - |
Aspergillus niger extract, pH 7.5 | Aspergillus niger |
33.5 | - |
purification step acetone precipitation | Aspergillus niger |
35.8 | - |
purification step Macro-Prep High Q | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ochratoxin A + H2O | - |
Aspergillus niger | ochratoxin alpha + L-beta-phenylalanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carboxypeptidase a | - |
Aspergillus niger |
ochratoxin A hydrolytic enzyme | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Aspergillus niger |